Erythrocyte survival is controlled by microRNA-142
نویسندگان
چکیده
Hematopoietic-specific microRNA-142 is a critical regulator of various blood cell lineages, but its role in erythrocytes is unexplored. Herein, we characterize the impact of microRNA-142 on erythrocyte physiology and molecular cell biology, using a mouse loss-of-function allele. We report that microRNA-142 is required for maintaining the typical erythrocyte biconcave shape and structural resilience, for the normal metabolism of reactive oxygen species, and for overall lifespan. microRNA-142 further controls ACTIN filament homeostasis and membrane skeleton organization. The analyses presented reveal previously unappreciated functions of microRNA-142 and contribute to an emerging view of small RNAs as key players in erythropoiesis. Finally, the work herein demonstrates how a housekeeping network of cytoskeletal regulators can be reshaped by a single micro-RNA denominator in a cell type specific manner.
منابع مشابه
Repression of miR-142 by p300 and MAPK is required for survival signalling via gp130 during adaptive hypertrophy
An increase in cardiac workload, ultimately resulting in hypertrophy, generates oxidative stress and therefore requires the activation of both survival and growth signal pathways. Here, we wanted to characterize the regulators, targets and mechanistic roles of miR-142, a microRNA (miRNA) negatively regulated during hypertrophy. We show that both miRNA-142-3p and -5p are repressed by serum-deriv...
متن کاملExpression of microRNA-142-3p in cervical cancer and its correlation with prognosis.
OBJECTIVE The aim of current study was to assess the association between miR-142-3p expression and the prognosis of patients with cervical cancer. PATIENTS AND METHODS Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to assess the expression level of miR-142-3p in cervical cancer tissues and matched normal tissues. Then, statistical analysis was performed to determine ...
متن کاملMiR-142-3p is a RANKL-dependent inducer of cell death in osteoclasts.
MicroRNA are small, non-coding, single-stranded RNAs that are estimated to regulate ~60% of the human genome. MiRNA profiling of monocyte-to-osteoclast differentiation identified miR-142-3p as a miRNA that is significantly, differentially expressed throughout osteoclastogenesis. Enforced expression of miR-142-3p via transient transfection with miR-142-3p mimic inhibited cell-to-cell contact and...
متن کاملDownregulation of Plasma MiR-142-3p and MiR-26a-5p in Patients With Colorectal Carcinoma
BACKGROUND Colorectal cancer is one of the most commonly diagnosed cancers and cancer- related death worldwide. Identification of new specific biomarkers could be helpful to detection of this malignancy. Altered plasma microRNA expression has been identified in many cancers, including colorectal cancer. OBJECTIVES The main objective of this study was to identify the circulating microRNAs with...
متن کاملmicroRNA-142-3p inhibits apoptosis and inflammation induced by bleomycin through down-regulation of Cox-2 in MLE-12 cells
microRNA (miR)-142-3p is implicated in malignancy and has been identified as a biomarker for aggressive and recurrent lung adenocarcinomas. This study aimed to evaluate the inhibitory effect of miR-142-3p on apoptosis and inflammation induced by bleomycin in MLE-12 cells. MLE-12 cells were first transfected either with miR-142-3p mimic or miR-142-3p inhibitor and then the cells were exposed to ...
متن کامل